Tzudiker attended the University of Colorado Boulder, where she earned her BFA in film and video. During her time there, she was also a part of the Feminist Student Network and Women's Resource Network. After graduating she attended Massachusetts College of Art and Design, where she earned her MFA in film and video.
After graduating MassArt, she spent two years teaching the fundamental of filmmaking there. She has worked for various companies and specializes in several computer programs.
She currently works as a production coordinator at Cartoon Network.
She is mainly known for her work on Gravity Falls but she also produced some shorts and video shorts campaigning presidential election and Barack Obama.